منابع مشابه
Fluorescent T7 display phages obtained by translational frameshift
Lytic phages form a powerful platform for the display of large cDNA libraries and offer the possibility to screen for interactions with almost any substrate. To visualize these interactions directly by fluorescence microscopy, we constructed fluorescent T7 phages by exploiting the flexibility of phages to incorporate modified versions of its capsid protein. By applying translational frameshift ...
متن کاملClassification of bacterial viruses; the relationship of two Serratia phages to coli-dysentery phages T3, T7, and D44.
Routine serological testing of a number of strains of bacteriophage indicated that some of them were related serologically to coliphages T3 and T7. One of these strains was dysentery phage D44, placed by Burnet (1933) in serological group 2 of the coli-dysentery phages. Two others were phage strains I and IV of the group of Serratia marcescens phages described by Wasermann and Seligmann (1953)....
متن کاملNegative control of protein synthesis after infection with bacteriophage T7.
T7 phage induces two negative control mechanisms of protein synthesis: (a) Host-gene expression is repressed by a "T7 repressor," and (b) early T7 protein synthesis is inhibited by a late phage protein.(a) The repressor for host enzyme synthesis is an early T7 protein. Its gene is none of the known early genes; it is located promotor-proximal to gene 1. The repressor function of this protein ca...
متن کاملThe T7-Related Pseudomonas putida Phage ϕ15 Displays Virion-Associated Biofilm Degradation Properties
Formation of a protected biofilm environment is recognized as one of the major causes of the increasing antibiotic resistance development and emphasizes the need to develop alternative antibacterial strategies, like phage therapy. This study investigates the in vitro degradation of single-species Pseudomonas putida biofilms, PpG1 and RD5PR2, by the novel phage ϕ15, a 'T7-like virus' with a viri...
متن کاملLysogenic Conversion and Phage Resistance Development in Phage Exposed Escherichia coli Biofilms
In this study, three-day old mature biofilms of Escherichia coli were exposed once to either a temperate Shiga-toxin encoding phage (H-19B) or an obligatory lytic phage (T7), after which further dynamics in the biofilm were monitored. As such, it was found that a single dose of H-19B could rapidly lead to a near complete lysogenization of the biofilm, with a subsequent continuous release of inf...
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ژورنال
عنوان ژورنال: Journal of Biotechnology
سال: 2007
ISSN: 0168-1656
DOI: 10.1016/j.jbiotec.2007.07.458